Clarity® HPLC/PREP Columns
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Each product in the Clarity BioSolutions portfolio has been designed to efficiently and effectively purify or characterize synthetic oligonucleotides used in biological research, therapeutic development and biochemical manufacturing.
- Easily separate N-1 failure sequences from target oligo with > 90% purities
- Trityl-off purification of DNA, RNA, Thioates, and modified/labeled oligonucleotides
- Preparative dimensions and particle sizes for loads > 5μmol
- Purify oligos up to 60 mer in length
- Excellent column for reversed phase HPLC quality control (QC) testing
LC-MS Analysis Test
Oligo-XT
An increased need for TK and PK/PD studies for oligo therapeutics has lead to an increase in LC-MS/MS analysis because of the specificity, sensitivity, and shorter method development times compared to ligand binding assays or ELISA. Because sensitivity is of the utmost importance, it is crucial to select an LC column that provides both separation and high efficiencies to ensure accurate results.
Clarity Core-Shell
Conventional Fully Porous
The Core-Shell Advantage
Unlike traditional fully porous oligo columns, Clarity Oligo-XT relies on the power of core-shell technology to provide extremely high efficiencies for both low and high oligo concentrations. Because the Clarity Oligo-XT particle is not fully porous, analytes spend less time diffusing into and out of the pores as they travel through the column, resulting in less band broadening for higher peak efficiencies.
*May not be representative of all application
Clarity Oligo-XT is patented by Phenomenex. U.S. Patent No. 7,563,367 and 8,658,038 and foreign counterparts.
Analytical LC abc
Clarity Oligo-RP™
Clarity Oligo-RP columns have been specifically designed for the reversed-phase purification of oligonucleotides with balanced hydrophobicity and polar selectivity. Available in 3, 5, and 10 µm particles, Clarity Oligo-RP allows for high-capacity analysis and purification of oligos.
A Highly Selective Analysis
Separation of failure N-1 from target N sequence
Column Clarity 3 µm Oligo-RP C18
Dimensions 50 x 4.6 mm
Part No. 00B-4441-E0
Mobile Phase
A: 50 mM TEAA pH 7.5
B: Methanol
Gradient 10 % to 45 % B in 30 minutes
Flow Rate 1 mL/ min
Detection UV @ 260 nm
Sample
1. 40nt DNA with sequence
CTTCTGAACAGTTGATCTATG-
CACTTCAGACTTATGATCA (2.5 μg)
2. 39nt DNA with sequence
TTCTGAACAGTTGATCTATGCAC-
TTCAGACTTATGATCA (2.5 μg)
Purification
DNA Purification
Clarity Oligo-RP columns allow for high loadability and deliver high recovery and purity, from HPLC to Preparative Purification.
Easily Scale from HPLC to Preparative Purification
Column Clarity 3 µm Oligo-RP C18
Dimensions
A: 50 x 10.0 mm
B: 50 x 4.6 mm
Part No.
A: 00B-4441-N0
B: 00B-4441-E0
Mobile Phase
A: 50 mM TEAA pH 7.5
B: Methanol
Gradient 10 % to 60 % B in 20 minutes
Flow Rate
A: 4.7 mL/ min
B: 1.0 mL/ min
Detection UV @ 260 nm
Sample 20nt DNA